Composite

Part:BBa_K1422504:Design

Designed by: Casey Martin   Group: iGEM14_Purdue   (2014-10-17)


Lactose-induced Killswitch using antiholin Hyb5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 457
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This killswitch is intended for Bacillus subtilis, and as such uses a RBS that works well in Bacillus. The choice of lactose as a promoter was chosen because it penetrates soil, is economically feasible to spread across a field, is not a food source for Bacillus, and does not harm plants or the natural soil microbiome. Hyb5 was chosen as a lysing agent because it is effective on Bacillus subtilis at neutral pH around field temperature.


Source

Hyb5 comes from NCBI NCBI. (2 December, 2008). Lactobacillus phage YB5 holin gene, complete cds. http://www.ncbi.nlm.nih.gov/nuccore/EF623894?report=GenBank

References